CATH Classification

Domain Context

CATH Clusters

Superfamily 3.30.70.370
Functional Family

Enzyme Information

2.7.7.6
DNA-directed RNA polymerase.
based on mapping to UniProt P00573
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
-!- Catalyzes DNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. -!- Can initiate a chain de novo. -!- In eukaryotes three forms of the enzyme have been distinguished on the basis of sensitivity of alpha-amanitin, and the type of RNA synthesized. -!- See also EC 2.7.7.19 and EC 2.7.7.48.

UniProtKB Entries (1)

P00573
RPOL_BPT7
Enterobacteria phage T7
T7 RNA polymerase

PDB Structure

PDB 2PI5
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Mechanism for de novo RNA synthesis and initiating nucleotide specificity by t7 RNA polymerase.
Kennedy, W.P., Momand, J.R., Yin, Y.W.
J.Mol.Biol.