CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.91.30
Functional Family

Enzyme Information

3.1.21.2
Deoxyribonuclease IV.
based on mapping to UniProt P00641
Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.
-!- The enzyme is an apurinic/apyrimidinic (AP) site endonuclease that primes DNA repair synthesis at AP sites. -!- It specifically cleaves the DNA backbone at AP sites and also removes 3' DNA-blocking groups such as 3' phosphates, 3' phosphoglycolates, and 3' alpha,beta-unsaturated aldehydes that arise from oxidative base damage and the activity of combined glycosylase/lyase enzymes. -!- It is also the only known repair enzyme that is able to cleave the DNA backbone 5' of the oxidative lesion alpha-deoxyadenosine. -!- The enzyme has a strong preference for single-stranded DNA. -!- Formerly EC 3.1.4.30.

UniProtKB Entries (1)

P00641
ENDO_BPT7
Enterobacteria phage T7
Endonuclease I

PDB Structure

PDB 2PFJ
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
The structural basis of Holliday junction resolution by T7 endonuclease I.
Hadden, J.M., Declais, A.C., Carr, S.B., Lilley, D.M., Phillips, S.E.
Nature