CATH Classification

Domain Context

CATH Clusters

Superfamily 3.30.160.20
Functional Family Ribonuclease 3

Enzyme Information

3.1.26.3
Ribonuclease III.
based on mapping to UniProt O67082
Endonucleolytic cleavage to 5'-phosphomonoester.
-!- An endoribonuclease that cleaves double-stranded RNA molecules. -!- The cleavage can be either a single-stranded nick or double-stranded break in the RNA, depending in part upon the degree of base-pairing in the region of the cleavage site. -!- Specificity is conferred by negative determinants, i.e., the presence of certain Watson-Crick base-pairs at specific positions that strongly inhibit cleavage. -!- RNase III is involved in both rRNA processing and mRNA processing and decay.

UniProtKB Entries (1)

O67082
RNC_AQUAE
Aquifex aeolicus VF5
Ribonuclease 3

PDB Structure

PDB 2NUG
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
A stepwise model for double-stranded RNA processing by ribonuclease III.
Gan, J., Shaw, G., Tropea, J.E., Waugh, D.S., Court, D.L., Ji, X.
Mol.Microbiol.