CATH Classification

Domain Context

CATH Clusters

Superfamily Phosphotransferase system, mannose-type IIA component
Functional Family PTS system mannose-specific transporter subunit IIAB

Enzyme Information

2.7.1.191
Protein-N(pi)-phosphohistidine--D-mannose phosphotransferase.
based on mapping to UniProt P69797
[Protein]-N(pi)-phospho-L-histidine + D-mannose(Side 1) = [protein]-L- histidine + D-mannose 6-phosphate(Side 2).
-!- This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). -!- The system, which is found only in prokaryotes, simultaneously transports its substrate from the periplasm or extracellular space into the cytoplasm and phosphorylates it. -!- The phosphate donor, which is shared among the different systems, is a phospho-carrier protein of low molecular mass that has been phosphorylated by EC 2.7.3.9. -!- Enzyme II, on the other hand, is specific for a particular substrate, although in some cases alternative substrates can be transported with lower efficiency. -!- The reaction involves a successive transfer of the phosphate group to several amino acids within the enzyme before the final transfer to the substrate. -!- Formerly EC 2.7.1.69.

UniProtKB Entries (1)

P69797
PTNAB_ECOLI
Escherichia coli K-12
PTS system mannose-specific EIIAB component

PDB Structure

PDB 2JZO
External Links
Method SOLUTION NMR
Organism Escherichia
Primary Citation
Solution NMR Structures of Productive and Non-productive Complexes between the A and B Domains of the Cytoplasmic Subunit of the Mannose Transporter of the Escherichia coli Phosphotransferase System.
Hu, J., Hu, K., Williams, D.C., Komlosh, M.E., Cai, M., Clore, G.M.
J.Biol.Chem.