CATH Classification

Domain Context

CATH Clusters

Superfamily Chitobiase/beta-hexosaminidase domain 2-like
Functional Family O-GlcNAcase NagJ

Enzyme Information

3.2.1.169
Protein O-GlcNAcase.
based on mapping to UniProt Q0TR53
(1) [Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H(2)O = [protein]-L-serine + N-acetyl-D-glucosamine. (2) [Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H(2)O = [protein]-L-threonine + N-acetyl-D-glucosamine.
-!- Within higher eukaryotes post-translational modification of protein serines/threonines with N-acetylglucosamine (O-GlcNAc) is dynamic, inducible and abundant, regulating many cellular processes by interfering with protein phosphorylation. -!- EC 2.4.1.155 transfers GlcNAc onto substrate proteins and EC 3.2.1.169 cleaves GlcNAc from the modified proteins.

UniProtKB Entries (1)

Q0TR53
OGA_CLOP1
Clostridium perfringens ATCC 13124
O-GlcNAcase NagJ

PDB Structure

PDB 2J62
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
GlcNAcstatin: a picomolar, selective O-GlcNAcase inhibitor that modulates intracellular O-glcNAcylation levels.
Dorfmueller, H.C., Borodkin, V.S., Schimpl, M., Shepherd, S.M., Shpiro, N.A., van Aalten, D.M.
J. Am. Chem. Soc.