CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.50.880
Functional Family Glutamine amidotransferase subunit pdxT, putative

Enzyme Information

3.5.1.2
Glutaminase.
based on mapping to UniProt Q9WYU3
L-glutamine + H(2)O = L-glutamate + NH(3).
4.3.3.6
Pyridoxal 5'-phosphate synthase (glutamine hydrolyzing).
based on mapping to UniProt Q9WYU3
D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H(2)O + phosphate.
-!- The ammonia is provided by the glutaminase subunit and channeled to the active site of the lyase subunit by a 100 A tunnel. -!- The enzyme can also use ribulose 5-phosphate and dihydroxyacetone phosphate. -!- The enzyme complex is found in aerobic bacteria, archeae, fungi and plants.

UniProtKB Entries (1)

Q9WYU3
PDXT_THEMA
Thermotoga maritima MSB8
Pyridoxal 5'-phosphate synthase subunit PdxT

PDB Structure

PDB 2ISS
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structural Insights into the Mechanism of the PLP Synthase Holoenzyme from Thermotoga maritima
Zein, F., Zhang, Y., Kang, Y.N., Burns, K., Begley, T.P., Ealick, S.E.
Biochemistry