CATH Classification

Domain Context

CATH Clusters

Superfamily HIV Type 1 Reverse Transcriptase, subunit A, domain 1
Functional Family Gag-Pol polyprotein

Enzyme Information

3.1.26.13
Retroviral ribonuclease H.
based on mapping to UniProt P03366
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
-!- Retroviral reverse transcriptase is a multifunctional enzyme responsible for viral replication. -!- To perform this task the enzyme combines two distinct activities. -!- The polymerase domain (EC 2.7.7.49) occupies the N-terminal two- thirds of the reverse transcriptase whereas the ribonuclease H domain comprises the C-terminal remaining one-third. -!- The RNase H domain of Moloney murine leukemia virus and Human immunodeficiency virus display two metal binding sites.
2.7.7.7
DNA-directed DNA polymerase.
based on mapping to UniProt P03366
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
-!- Catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time. -!- Cannot initiate a chain de novo. -!- Requires a primer which may be DNA or RNA. -!- See also EC 2.7.7.49.
2.7.7.-
Nucleotidyltransferases.
based on mapping to UniProt P03366
2.7.7.49
RNA-directed DNA polymerase.
based on mapping to UniProt P03366
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
-!- Catalyzes RNA-template-directed extension of the 3'-end of a DNA strand by one deoxynucleotide at a time. -!- Cannot initiate a chain de novo. -!- Requires a RNA or DNA primer. -!- DNA can also serve as template. -!- See also EC 2.7.7.7.
3.1.-.-
Acting on ester bonds.
based on mapping to UniProt P03366
3.1.13.2
Exoribonuclease H.
based on mapping to UniProt P03366
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
-!- This is a secondary reaction to the RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end performed by EC 3.1.26.13.
3.4.23.16
HIV-1 retropepsin.
based on mapping to UniProt P03366
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
-!- Present in human immunodeficiency virus type 1. -!- Contributes to the maturation of the viral particle, and is a target of antiviral drugs. -!- Active enzyme is a dimer of identical 11-kDa subunits. -!- Similar enzymes occur in other retroviruses. -!- Belongs to peptidase family A2.

UniProtKB Entries (1)

P03366
POL_HV1B1
Human immunodeficiency virus type 1 BH10
Gag-Pol polyprotein

PDB Structure

PDB 2IAJ
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal Structures of Clinically Relevant Lys103Asn/Tyr181Cys Double Mutant HIV-1 Reverse Transcriptase in Complexes with ATP and Non-nucleoside Inhibitor HBY 097.
Das, K., Sarafianos, S.G., Clark, A.D., Boyer, P.L., Hughes, S.H., Arnold, E.
J.Mol.Biol.