CATH Classification

Domain Context

CATH Clusters

Superfamily 3.30.70.3180
Functional Family

Enzyme Information

2.1.1.148
Thymidylate synthase (FAD).
based on mapping to UniProt P9WG57
5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP(+).
-!- Contains FAD. -!- All thymidylate synthases catalyze a reductive methylation involving the transfer of the methylene group of 5,10-methylenetetrahydrofolate to the C5-position of dUMP and a two electron reduction of the methylene group to a methyl group. -!- Unlike the classical thymidylate synthase, ThyA (EC 2.1.1.45), which uses folate as both a 1-carbon donor and a source of reducing equivalents, this enzyme uses a flavin coenzyme as a source of reducing equivalents, which are derived from NADPH.

UniProtKB Entries (1)

P9WG57
THYX_MYCTU
Mycobacterium tuberculosis H37Rv
Flavin-dependent thymidylate synthase

PDB Structure

PDB 2GQ2
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
NADP+ expels both the co-factor and a substrate analog from the Mycobacterium tuberculosis ThyX active site: opportunities for anti-bacterial drug design.
Sampathkumar, P., Turley, S., Sibley, C.H., Hol, W.G.
J.Mol.Biol.