CATH Classification

Domain Context

CATH Clusters

Superfamily Poxvirus poly(A) polymerase, nucleotidyltransferase domain
Functional Family

Enzyme Information

2.7.7.19
Polynucleotide adenylyltransferase.
based on mapping to UniProt P23371
ATP + RNA(n) = diphosphate + RNA(n+1).
-!- Also acts slowly with CTP. -!- Catalyzes template-independent extension of the 3'-end of a DNA strand by one nucleotide at a time. -!- Cannot initiate a chain de novo. -!- The primer, depending on the source of the enzyme, may be an RNA or DNA fragment or oligo(A) bearing a 3'-OH terminal group. -!- See also EC 2.7.7.6.

UniProtKB Entries (2)

Q1PIV4
Q1PIV4_9POXV
Vaccinia virus
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase
P23371
PAP1_VACCW
Vaccinia virus WR
Poly(A) polymerase catalytic subunit

PDB Structure

PDB 2GAF
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal structures of the vaccinia virus polyadenylate polymerase heterodimer: insights into ATP selectivity and processivity.
Moure, C.M., Bowman, B.R., Gershon, P.D., Quiocho, F.A.
Mol.Cell