CATH Classification

Domain Context

CATH Clusters

Superfamily Peptide N glycanase, PAW domain
Functional Family Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

Enzyme Information

3.5.1.52
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase.
based on mapping to UniProt Q9JI78
Hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue.
-!- Does not act on (GlcNAc)-Asn, because it requires the presence of more than two amino-acid residues in the substrate (cf. EC 3.5.1.26). -!- Formerly EC 3.2.2.18.

UniProtKB Entries (1)

Q9JI78
NGLY1_MOUSE
Mus musculus
Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase

PDB Structure

PDB 2G9F
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structural and biochemical studies of the C-terminal domain of mouse peptide-N-glycanase identify it as a mannose-binding module.
Zhou, X., Zhao, G., Truglio, J.J., Wang, L., Li, G., Lennarz, W.J., Schindelin, H.
Proc.Natl.Acad.Sci.Usa