CATH Classification

Domain Context

CATH Clusters

Superfamily 1.50.10.10
Functional Family

Enzyme Information

3.2.1.179
Gellan tetrasaccharide unsaturated glucuronosyl hydrolase.
based on mapping to UniProt Q9RC92
Beta-D-4-deoxy-Delta(4)-GlcAp-(1->4)-beta-D-Glcp-(1->4)-alpha-L-Rhap- (1->3)-D-Glcp + H(2)O = 5-dehydro-4-deoxy-D-glucuronate + beta-D-Glcp- (1->4)-alpha-L-Rhap-(1->3)-D-Glcp.
-!- The enzyme releases 4-deoxy-4(5)-unsaturated D-glucuronic acid from oligosaccharides produced by polysaccharide lyases, e.g. the tetrasaccharide beta-D-4-deoxy-Delta(4)-GlcAp-(1->4)-beta-D-Glcp- (1->4)-alpha-L-Rhap-(1->3)-D-Glcp produced by EC 4.2.2.25. -!- The enzyme can also hydrolyze unsaturated chondroitin and hyaluronate disaccharides (beta-D-4-deoxy-Delta(4)-GlcAp-(1->3)-beta-D-GalNAc, beta-D-4-deoxy-Delta(4)-GlcAp-(1->3)-beta-D-GalNAc6S, beta-D-4-deoxy- Delta(4)-GlcAp2S-(1->3)-beta-D-GalNAc, beta-D-4-deoxy-Delta(4)-GlcAp- (1->3)-beta-D-GlcNAc), preferring the unsulfated disaccharides to the sulfated disaccharides.

UniProtKB Entries (1)

Q9RC92
UGL_BACGL
Bacillus sp. GL1
Unsaturated glucuronyl hydrolase

PDB Structure

PDB 2FV0
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp. GL1
Itoh, T., Hashimoto, W., Mikami, B., Murata, K.
Biochem.Biophys.Res.Commun.