CATH Classification

Domain Context

CATH Clusters

Superfamily ACP-like
Functional Family Isochorismatase

Enzyme Information

6.3.2.14
Enterobactin synthase.
based on mapping to UniProt P0ADI4
6 ATP + 3 2,3-dihydroxybenzoate + 3 L-serine = enterobactin + 6 AMP + 6 diphosphate.
-!- This enzyme complex catalyzes the conversion of three molecules each of 2,3-dihydroxybenzoate and L-serine to form the siderophore enterobactin. -!- In Escherichia coli the complex is formed by EntB (an aryl carrier protein that has to be activated by 4'-phosphopantetheine), EntD (a phosphopantetheinyl transferase that activates EntB), EntE (catalyzes the ATP-dependent condensation of 2,3-dihydroxybenzoate and holo-EntB to form the covalently arylated form of EntB), and EntF (a four domain protein that catalyzes the activation of L-serine by ATP, the condensation of the activated L-serine with the activated 2,3- dihydroxybenzoate, and the trimerization of three such moieties to a single enterobactin molecule).
3.3.2.1
Isochorismatase.
based on mapping to UniProt P0ADI4
Isochorismate + H(2)O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate.

UniProtKB Entries (1)

P0ADI4
ENTB_ECOLI
Escherichia coli K-12
Enterobactin synthase component B

PDB Structure

PDB 2FQ1
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structure of the EntB multidomain nonribosomal peptide synthetase and functional analysis of its interaction with the EntE adenylation domain.
Drake, E.J., Nicolai, D.A., Gulick, A.M.
Chem.Biol.