CATH Classification

Domain Context

CATH Clusters

Superfamily T4 endonuclease V
Functional Family Endonuclease V

Enzyme Information

4.2.99.18
DNA-(apurinic or apyrimidinic site) lyase.
based on mapping to UniProt P04418
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
-!- 'Nicking' of the phosphodiester bond is due to a lyase-type reaction, not hydrolysis. -!- This group of enzymes was previously listed as endonucleases, under the number EC 3.1.25.2.
3.2.2.17
Deoxyribodipyrimidine endonucleosidase.
based on mapping to UniProt P04418
Cleaves the N-glycosidic bond between the 5'-pyrimidine residue in cyclobutadipyrimidine (in DNA) and the corresponding deoxy-D-ribose residue.

UniProtKB Entries (1)

P04418
END5_BPT4
Escherichia virus T4
Endonuclease V

PDB Structure

PDB 2FCC
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structure of T4 Pyrimidine Dimer Glycosylase in a Reduced Imine Covalent Complex with Abasic Site-containing DNA.
Golan, G., Zharkov, D.O., Grollman, A.P., Dodson, M.L., McCullough, A.K., Lloyd, R.S., Shoham, G.
J.Mol.Biol.