CATH Classification

Domain Context

CATH Clusters

Superfamily TPP-binding domain
Functional Family Pyruvate oxidase

Enzyme Information

1.2.3.3
Pyruvate oxidase.
based on mapping to UniProt P37063
Pyruvate + phosphate + O(2) = acetyl phosphate + CO(2) + H(2)O(2).
-!- Two reducing equivalents are transferred from the resonant carbanion/enamine forms of 2-hydroxyethyl-thiamine-diphosphate to the adjacent flavin cofactor, yielding 2-acetyl-thiamine diphosphate (AcThDP) and reduced flavin. -!- FADH is reoxidized by O(2) to yield H(2)O(2) and FAD and AcThDP is cleaved phosphorolytically to acetyl phosphate and thiamine diphosphate.

UniProtKB Entries (1)

P37063
POXB_LACPL
Lactobacillus plantarum WCFS1
Pyruvate oxidase

PDB Structure

PDB 2EZ4
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
The catalytic cycle of a thiamin diphosphate enzyme examined by cryocrystallography.
Wille, G., Meyer, D., Steinmetz, A., Hinze, E., Golbik, R., Tittmann, K.
Nat.Chem.Biol.