CATH Classification

Domain Context

CATH Clusters

Functional Family Lipid A deacylase

Enzyme Information
Acyloxyacyl hydrolase.
based on mapping to UniProt Q9HVD1
3-(acyloxy)acyl group of bacterial toxin = 3-hydroxyacyl group of bacterial toxin + a fatty acid.
-!- The substrate is lipid A on the reducing end of the toxic lipopolysaccharide (LPS) of Salmonella typhimurium and related organisms. -!- It consists of diglucosamine, beta-D-GlcN-(1->6)-D-GlcN, attached by glycosylation on O-6 of its non-reducing residue, phosphorylated on O-4 of this residue and on O-1 of its potentially reducing residue. -!- Both residues carry 3-(acyloxy)acyl groups on N-2 and O-3. -!- The enzyme from human leukocytes detoxifies the lipid by hydrolyzing the secondary acyl groups from O-3 of the 3-hydroxyacyl groups on the disaccharide (LPS). -!- It also possesses a wide range of phospholipase and acyltransferase activities (e.g. EC, EC, EC and EC, hydrolyzing diacylglycerol and phosphatidyl compounds, but not triacylglycerols. -!- It has a preference for saturated C(12)-C(16) acyl groups.

UniProtKB Entries (1)

Pseudomonas aeruginosa PAO1
Lipid A deacylase PagL

PDB Structure

External Links
Organism Escherichia
Primary Citation
Crystal structure and catalytic mechanism of the LPS 3-O-deacylase PagL from Pseudomonas aeruginosa.
Rutten, L., Geurtsen, J., Lambert, W., Smolenaers, J.J., Bonvin, A.M., de Haan, A., van der Ley, P., Egmond, M.R., Gros, P., Tommassen, J.