CATH Classification

Domain Context

CATH Clusters

Superfamily 2.40.40.20
Functional Family Probable DNA-directed RNA polymerase

Enzyme Information

2.7.7.6
DNA-directed RNA polymerase.
based on mapping to UniProt P04050
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
-!- Catalyzes DNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. -!- Can initiate a chain de novo. -!- In eukaryotes three forms of the enzyme have been distinguished on the basis of sensitivity of alpha-amanitin, and the type of RNA synthesized. -!- See also EC 2.7.7.19 and EC 2.7.7.48.

UniProtKB Entries (1)

P40422
RPAB4_YEAST
Saccharomyces cerevisiae S288C
DNA-directed RNA polymerases I, II, and III subunit RPABC4

PDB Structure

PDB 2E2I
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural basis of transcription: role of the trigger loop in substrate specificity and catalysis
Wang, D., Bushnell, D.A., Westover, K.D., Kaplan, C.D., Kornberg, R.D.
Cell(Cambridge,Mass.)