CATH Domain 2dj5B00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.40	3-Layer(aba) Sandwich
	3.40.50	Rossmann fold
	3.40.50.1400

Domain Context

CATH Clusters

Superfamily	3.40.50.1400
Functional Family	Sirohydrochlorin cobaltochelatase

Enzyme Information

4.99.1.3

Sirohydrochlorin cobaltochelatase.

based on mapping to UniProt O29537

Cobalt-sirohydrochlorin + 2 H(+) = sirohydrochlorin + Co(2+).

-!- This enzyme is a type II chelatase, being either a monomer (CbiX) or a homodimer (CibK) and being ATP-independent. -!- CbiK from Salmonella enterica uses precorrin-2 as the substrate to yield cobalt-precorrin-2. -!- The enzyme contains two histidines at the active site that are thought to be involved in the deprotonation of the tetrapyrrole substrate as well as in metal binding. -!- CbiX from Bacillus megaterium inserts cobalt at the level of sirohydrochlorin (factor-II) rather than precorrin-2.

UniProtKB Entries (1)

O29537

CBIX_ARCFU

Archaeoglobus fulgidus DSM 4304

Sirohydrochlorin cobaltochelatase

PDB Structure

PDB	2DJ5
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism	Escherichia
Primary Citation	Crystal Structure of the Vitamin B(12) Biosynthetic Cobaltochelatase, CbiX (S), from Archaeoglobus Fulgidus Yin, J., Xu, L.X., Cherney, M.M., Raux-Deery, E., Bindley, A.A., Savchenko, A., Walker, J.R., Cuff, M.E., Warren, M.J., James, M.N.G. J.STRUCT.FUNCT.GENOM.