CATH Classification

Domain Context

CATH Clusters

Superfamily Trypsin-like serine proteases
Functional Family Genome polyprotein

Enzyme Information

3.4.22.28
Picornain 3C.
based on mapping to UniProt P13901
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
-!- From entero-, rhino-, aphto- and cardioviruses. -!- Larger than the homologous virus picornain 2A. -!- Belongs to peptidase family C3.
3.6.1.15
Nucleoside-triphosphate phosphatase.
based on mapping to UniProt P13901
NTP + H(2)O = NDP + phosphate.
-!- The enzyme is found in eukaryotes and thermophilic bacteria, but appears to be absent from mesophilic bacteria. -!- Also hydrolyzes nucleoside diphosphates, thiamine diphosphate and FAD. -!- The enzyme from the plant Pisum sativum (garden pea) is regulated by calmodulin.
2.7.7.48
RNA-directed RNA polymerase.
based on mapping to UniProt P13901
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
-!- Catalyzes RNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. -!- Can initiate a chain de novo. -!- See also EC 2.7.7.6.

UniProtKB Entries (1)

P13901
POLG_HAVMB
Human hepatitis A virus Hu/Northern Africa/MBB/1978
Genome polyprotein

PDB Structure

PDB 2CXV
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Dual Modes of Modification of Hepatitis A Virus 3C Protease by a Serine-derived beta-Lactone: Selective Crystallization and Formation of a Functional Catalytic Triad in the Active Site
Yin, J., Bergmann, E.M., Cherney, M.M., Lall, M.S., Jain, R.P., Vederas, J.C., James, M.N.G.
J.MOL.BIOL.