CATH Domain 2cfaB01

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.30	2-Layer Sandwich
	3.30.1360	Gyrase A; domain 2
	3.30.1360.170

Domain Context

CATH Clusters

Superfamily	3.30.1360.170
Functional Family

Enzyme Information

2.1.1.148

Thymidylate synthase (FAD).

based on mapping to UniProt O41156

5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP(+).

-!- Contains FAD. -!- All thymidylate synthases catalyze a reductive methylation involving the transfer of the methylene group of 5,10-methylenetetrahydrofolate to the C5-position of dUMP and a two electron reduction of the methylene group to a methyl group. -!- Unlike the classical thymidylate synthase, ThyA (EC 2.1.1.45), which uses folate as both a 1-carbon donor and a source of reducing equivalents, this enzyme uses a flavin coenzyme as a source of reducing equivalents, which are derived from NADPH.

UniProtKB Entries (1)

O41156

THYX_PBCV1

Paramecium bursaria Chlorella virus 1

Probable flavin-dependent thymidylate synthase

PDB Structure

PDB	2CFA
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Catalytic Mechanism and Structure of Viral Flavin-Dependent Thymidylate Synthase Thyx. Graziani, S., Bernauer, J., Skouloubris, S., Graille, M., Zhou, C.-Z., Marchand, C., Decottignies, P., Van Tilbeurgh, H., Myllykallio, H., Liebl, U. J.Biol.Chem.