CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.47.10
Functional Family Peroxisomal 3-ketoacyl-CoA thiolase

Enzyme Information

2.3.1.16
Acetyl-CoA C-acyltransferase.
based on mapping to UniProt Q56WD9
Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.
-!- The enzyme, found in both eukaryotes and in prokaryotes, is involved in degradation pathways such as fatty acid beta-oxidation. -!- The enzyme acts on 3-oxoacyl-CoAs to produce acetyl-CoA and an acyl- CoA shortened by two carbon atoms. -!- The reaction starts with the acylation of a nucleophilic cysteine at the active site by a 3-oxoacyl-CoA, with the concomitant release of acetyl-CoA. -!- In the second step the acyl group is transferred to CoA. -!- Most enzymes have a broad substrate range for the 3-oxoacyl-CoA. -!- cf. EC 2.3.1.9.

UniProtKB Entries (1)

Q56WD9
THIK2_ARATH
Arabidopsis thaliana
3-ketoacyl-CoA thiolase 2, peroxisomal

PDB Structure

PDB 2C7Z
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
The Crystal Structure of a Plant 3-Ketoacyl-Coa Thiolase Reveals the Potential for Redox Control of Peroxisomal Fatty Acid Beta-Oxidation.
Sundaramoorthy, R., Micossi, E., Alphey, M.S., Germain, V., Bryce, J.H., Smith, S.M., Leonard, G.A., Hunter, W.N.
J.Mol.Biol.