CATH Classification

Domain Context

CATH Clusters

Superfamily Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)
Functional Family Endonuclease III

Enzyme Information

4.2.99.18
DNA-(apurinic or apyrimidinic site) lyase.
based on mapping to UniProt P0AB83
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
-!- 'Nicking' of the phosphodiester bond is due to a lyase-type reaction, not hydrolysis. -!- This group of enzymes was previously listed as endonucleases, under the number EC 3.1.25.2.

UniProtKB Entries (1)

P0AB83
END3_ECOLI
Escherichia coli K-12
Endonuclease III

PDB Structure

PDB 2ABK
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Novel DNA binding motifs in the DNA repair enzyme endonuclease III crystal structure.
Thayer, M.M., Ahern, H., Xing, D., Cunningham, R.P., Tainer, J.A.
EMBO J.