CATH Classification

Domain Context

CATH Clusters

Superfamily Nitric Oxide Synthase;Heme Domain;Chain A domain 2
Functional Family Nitric oxide synthase, endothelial

Enzyme Information

1.14.13.39
Nitric-oxide synthase (NADPH).
based on mapping to UniProt P29473
2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.
-!- This eukaryotic enzyme, which is found in plants and animals, consists of oxygenase and reductase domains that are linked via a regulatory calmodulin-binding domain. -!- Upon calcium-induced calmodulin binding, the reductase and oxygenase domains form a complex, allowing electrons to flow from NADPH via FAD and FMN to the active center. -!- May produce superoxide under certain conditions. -!- Cf. EC 1.14.13.165.

UniProtKB Entries (1)

P29473
NOS3_BOVIN
Bos taurus
Nitric oxide synthase, endothelial

PDB Structure

PDB 1ZZS
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Exploring the Binding Conformations of Bulkier Dipeptide Amide Inhibitors in Constitutive Nitric Oxide Synthases.
Li, H., Flinspach, M.L., Igarashi, J., Jamal, J., Yang, W., Litzinger, E.A., Huang, H., Erdal, E.P., Silverman, R.B., Poulos, T.L.
Biochemistry