CATH Classification

Domain Context

CATH Clusters

Superfamily Ubiquitin Conjugating Enzyme
Functional Family ubiquitin-conjugating enzyme E2 U isoform X1

Enzyme Information

2.3.2.23
E2 ubiquitin-conjugating enzyme.
based on mapping to UniProt Q5VVX9
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine.
-!- The E2 ubiquitin-conjugating enzyme acquires the activated ubquitin from the E1 ubiquitin-activating enzyme (EC 6.2.1.45) and binds it via a transthioesterification reaction to itself. -!- In the human enzyme the catalytic center is located at Cys-87 where ubiquitin is bound via its C-terminal glycine in a thioester linkage. -!- Formerly EC 6.3.2.19.

UniProtKB Entries (1)

Q5VVX9
UBE2U_HUMAN
Homo sapiens
Ubiquitin-conjugating enzyme E2 U

PDB Structure

PDB 1YRV
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen.
Sheng, Y., Hong, J.H., Doherty, R., Srikumar, T., Shloush, J., Avvakumov, G.V., Walker, J.R., Xue, S., Neculai, D., Wan, J.W., Kim, S.K., Arrowsmith, C.H., Raught, B., Dhe-Paganon, S.
Mol Cell Proteomics