CATH Classification

Domain Context

CATH Clusters

Superfamily Diphtheria toxin, translocation domain
Functional Family Diphtheria toxin

Enzyme Information

2.4.2.36
NAD(+)--diphthamide ADP-ribosyltransferase.
based on mapping to UniProt P00588
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2].
-!- Dihtheria toxin and some other bacterial toxins catalyze this reaction, which inactivates translation elongation factor 2 (EF2). -!- The acceptor is diphthamide, a unique modification of a histidine residue in the elongation factor found in archaebacteria and all eukaryotes, but not in eubacteria. -!- Cf. EC 2.4.2.31. -!- The relevant histidine of EF2 is His(715) in mammals, His(699) in yeast and His(600) in Pyrococcus horikoshii.

UniProtKB Entries (2)

P00588
DTX_CORBE
Corynephage beta
Diphtheria toxin
Q99075
HBEGF_HUMAN
Homo sapiens
Proheparin-binding EGF-like growth factor

PDB Structure

PDB 1XDT
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal structure of the complex of diphtheria toxin with an extracellular fragment of its receptor.
Louie, G.V., Yang, W., Bowman, M.E., Choe, S.
Mol.Cell