CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.1280.10
Functional Family RNA methyltransferase, TrmH family

Enzyme Information

2.1.1.208
23S rRNA (uridine(2479)-2'-O)-methyltransferase.
based on mapping to UniProt Q9F5K6
S-adenosyl-L-methionine + uridine(2479) in 23S rRNA = S-adenosyl-L- homocysteine + 2'-O-methyluridine(2479) in 23S rRNA.
-!- Streptomyces viridochromogenes produces the antibiotic avilamycin A which binds to the 50S ribosomal subunit to inhibit protein synthesis. -!- To protect itself from the antibiotic, Streptomyces viridochromogenes utilizes two methyltransferases, 23S rRNA (uridine(2479)-2'-O)- methyltransferase and EC 2.1.1.209 whose actions confer avilamycin resistance to the RNA.

UniProtKB Entries (1)

Q9F5K6
AVRB_STRVR
Streptomyces viridochromogenes
23S rRNA (uridine(2479)-2'-O)-methyltransferase

PDB Structure

PDB 1X7P
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structure and function of the antibiotic resistance-mediating methyltransferase AviRb from Streptomyces viridochromogenes
Mosbacher, T.G., Bechthold, A., Schulz, G.E.
J.Mol.Biol.