CATH Classification

Domain Context

CATH Clusters

Superfamily Rhodanese-like domain
Functional Family Sulfurtransferase

Enzyme Information

2.8.1.2
3-mercaptopyruvate sulfurtransferase.
based on mapping to UniProt P31142
3-mercaptopyruvate + reduced thioredoxin = pyruvate + hydrogen sulfide + oxidized thioredoxin.
-!- The enzyme catalyzes a transsulfuration reaction from 3-mercaptopyruvate to an internal cysteine residue. -!- In the presence of a dithiol such as reduced thioredoxin or dihydrolipoate, the sulfanyl sulfur is released as hydrogen sulfide. -!- The enzyme participates in a sulfur relay process that leads to the 2-thiolation of some tRNAs and to protein urmylation by transferring sulfur between the NFS1 cysteine desulfurase (EC 2.8.1.7) and the MOCS3 sulfurtransferase (EC 2.8.1.11).

UniProtKB Entries (1)

P31142
THTM_ECOLI
Escherichia coli K-12
3-mercaptopyruvate sulfurtransferase

PDB Structure

PDB 1URH
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
The "Rhodanese" Fold and Catalytic Mechanism of 3-Mercaptopyruvate Sulfurtransferases: Crystal Structure of Ssea from Escherichia Coli
Spallarossa, A., Forlani, F., Carpen, A., Armirotti, A., Pagani, S., Bolognesi, M., Bordo, D.
J.Mol.Biol.