CATH Classification

Domain Context

CATH Clusters

Superfamily Rieske [2Fe-2S] iron-sulphur domain
Functional Family

Enzyme Information

1.14.12.18
Biphenyl 2,3-dioxygenase.
based on mapping to UniProt Q53122
Biphenyl + NADH + O(2) = (1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD(+).
-!- The enzyme from Burkholderia fungorum strain LB400 (previously Pseudomonas sp.) is part of a multicomponent system composed of an NADH:ferredoxin oxidoreductase (FAD cofactor), a [2Fe-2S] Rieske-type ferredoxin, and a terminal oxygenase that contains a [2Fe-2S] Rieske- type iron-sulfur cluster and a catalytic mononuclear nonheme iron center. -!- Chlorine-substituted biphenyls can also act as substrates. -!- Similar to the three-component enzyme systems EC 1.14.12.3 and EC 1.14.12.11.

UniProtKB Entries (1)

Q53122
BPHA1_RHOJR
Rhodococcus jostii RHA1
Biphenyl 2,3-dioxygenase subunit alpha

PDB Structure

PDB 1ULI
External Links
Method X-RAY DIFFRACTION
Organism Rhodococcus
Primary Citation
Crystal Structure of the Terminal Oxygenase Component of Biphenyl Dioxygenase Derived from Rhodococcus sp. Strain RHA1
Furusawa, Y., Nagarajan, V., Tanokura, M., Masai, E., Fukuda, M., Senda, T.
J.Mol.Biol.