CATH Classification

Domain Context

CATH Clusters

Superfamily Uracil-DNA glycosylase-like domain
Functional Family Type-4 uracil-DNA glycosylase

Enzyme Information

3.2.2.27
Uracil-DNA glycosylase.
based on mapping to UniProt Q5SKC5
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.
-!- Uracil-DNA glycosylases are widespread enzymes that are found in all living organisms. -!- Uracil-DNA glycosylase (EC 3.2.2.27) and double-stranded uracil-DNA glycosylase (EC 3.2.2.28) form a central part of the DNA-repair machinery since they initiate the DNA base-excision repair pathway by hydrolyzing the N-glycosidic bond between uracil and the deoxyribose sugar thereby catalyzing the removal of mis-incorporated uracil from DNA.

UniProtKB Entries (1)

Q5SKC5
UDGA_THET8
Thermus thermophilus HB8
Type-4 uracil-DNA glycosylase

PDB Structure

PDB 1UI1
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal Structure of a Family 4 Uracil-DNA Glycosylase from Thermus thermophilus HB8
Hoseki, J., Okamoto, A., Masui, R., Shibata, T., Inoue, Y., Yokoyama, S., Kuramitsu, S.
J.Mol.Biol.