CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.50.360
Functional Family Nitric oxide synthase, endothelial

Enzyme Information

1.14.13.39
Nitric-oxide synthase (NADPH).
based on mapping to UniProt P29476
2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.
-!- This eukaryotic enzyme, which is found in plants and animals, consists of oxygenase and reductase domains that are linked via a regulatory calmodulin-binding domain. -!- Upon calcium-induced calmodulin binding, the reductase and oxygenase domains form a complex, allowing electrons to flow from NADPH via FAD and FMN to the active center. -!- May produce superoxide under certain conditions. -!- Cf. EC 1.14.13.165.

UniProtKB Entries (1)

P29476
NOS1_RAT
Rattus norvegicus
Nitric oxide synthase, brain

PDB Structure

PDB 1TLL
External Links
Method X-RAY DIFFRACTION
Organism Pichia
Primary Citation
Structural basis for isozyme-specific regulation of electron transfer in nitric-oxide synthase
Garcin, E.D., Bruns, C.M., Lloyd, S.J., Hosfield, D.J., Tiso, M., Gachhui, R., Stuehr, D.J., Tainer, J.A., Getzoff, E.D.
J.Biol.Chem.