CATH Classification

Domain Context

CATH Clusters

Superfamily Peptidase S8/S53 domain
Functional Family Tk-subtilisin

Enzyme Information

3.4.21.62
Subtilisin.
based on mapping to UniProt P00782
Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.
-!- Subtilisin is a serine endopeptidase that evolved independently of chymotrypsin. -!- It contains no cysteine residues (although these are found in homologous enzymes). -!- Species variants include subtilisin BPN' (also subtilisin B, subtilopeptidase C, nagarse, nagarse proteinase, subtilisin Novo, bacterial proteinase Novo) and subtilisin Carlsberg (subtilisin A, subtilopeptidase A, alcalase Novo). -!- Similar enzymes are produced by various Bacillus subtilis strains and other Bacillus species. -!- Belongs to peptidase family S8. -!- Formerly EC 3.4.4.16 and EC 3.4.21.14.

UniProtKB Entries (1)

P00782
SUBT_BACAM
Bacillus amyloliquefaciens
Subtilisin BPN'

PDB Structure

PDB 1S01
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding.
Pantoliano, M.W., Whitlow, M., Wood, J.F., Dodd, S.W., Hardman, K.D., Rollence, M.L., Bryan, P.N.
Biochemistry