CATH Classification

CATH Clusters

Superfamily DNA mismatch repair MutH/Restriction endonuclease, type II
Functional Family

Enzyme Information

3.1.21.4
Type II site-specific deoxyribonuclease.
based on mapping to UniProt P04390
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
-!- Large group of enzymes which recognize specific short DNA sequences and cleave either within, or at a short specific distance from, the recognition site. -!- See the REBASE database for a complete list of these enzymes: http://rebase.neb.com/rebase/

UniProtKB Entries (1)

P04390
T2E5_ECOLX
Escherichia coli
Type-2 restriction enzyme EcoRV

PDB Structure

PDB 1RV5
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Role of protein-induced bending in the specificity of DNA recognition: crystal structure of EcoRV endonuclease complexed with d(AAAGAT) + d(ATCTT).
Horton, N.C., Perona, J.J.
J.Mol.Biol.