CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.50.1400
Functional Family Sirohydrochlorin cobaltochelatase

Enzyme Information

4.99.1.3
Sirohydrochlorin cobaltochelatase.
based on mapping to UniProt Q05592
Cobalt-sirohydrochlorin + 2 H(+) = sirohydrochlorin + Co(2+).
-!- This enzyme is a type II chelatase, being either a monomer (CbiX) or a homodimer (CibK) and being ATP-independent. -!- CbiK from Salmonella enterica uses precorrin-2 as the substrate to yield cobalt-precorrin-2. -!- The enzyme contains two histidines at the active site that are thought to be involved in the deprotonation of the tetrapyrrole substrate as well as in metal binding. -!- CbiX from Bacillus megaterium inserts cobalt at the level of sirohydrochlorin (factor-II) rather than precorrin-2.

UniProtKB Entries (1)

Q05592
CBIK_SALTY
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Sirohydrochlorin cobaltochelatase

PDB Structure

PDB 1QGO
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Common chelatase design in the branched tetrapyrrole pathways of heme and anaerobic cobalamin synthesis.
Schubert, H.L., Raux, E., Wilson, K.S., Warren, M.J.
Biochemistry