CATH Classification

Domain Context

CATH Clusters

Superfamily 1.10.8.50
Functional Family Endonuclease 8

Enzyme Information

4.2.99.18
DNA-(apurinic or apyrimidinic site) lyase.
based on mapping to UniProt P50465
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
-!- 'Nicking' of the phosphodiester bond is due to a lyase-type reaction, not hydrolysis. -!- This group of enzymes was previously listed as endonucleases, under the number EC 3.1.25.2.
3.2.2.-
Hydrolyzing N-glycosyl compounds.
based on mapping to UniProt P50465

UniProtKB Entries (1)

P50465
END8_ECOLI
Escherichia coli K-12
Endonuclease 8

PDB Structure

PDB 1Q3C
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structure of the uncomplexed DNA repair enzyme endonuclease VIII indicates significant interdomain flexibility.
Golan, G., Zharkov, D.O., Feinberg, H., Fernandes, A.S., Zaika, E.I., Kycia, J.H., Grollman, A.P., Shoham, G.
Nucleic Acids Res.