CATH Classification

Domain Context

CATH Clusters

Superfamily Cytochrome c-like domain
Functional Family Nitrite reductase

Enzyme Information

1.7.99.1
Hydroxylamine reductase.
based on mapping to UniProt P24474
NH(3) + H(2)O + acceptor = hydroxylamine + reduced acceptor.
-!- Reduced pyocyanine, methylene blue and flavins act as donors for the reduction of hydroxylamine. -!- May be identical to EC 1.7.2.1.
1.7.2.1
Nitrite reductase (NO-forming).
based on mapping to UniProt P24474
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+).
-!- The reaction is catalyzed by two types of enzymes, found in the perimplasm of denitrifying bacteria. -!- One type comprises proteins containing multiple copper centers, the other a heme protein, cytochrome cd1. -!- Acceptors include c-type cytochromes such as cytochrome c-550 or cytochrome c-551 from Paracoccus denitrificans or Pseudomonas aeruginosa, and small blue copper proteins such as azurin and pseudoazurin. -!- Cytochrome cd1 also has oxidase and hydroxylamine reductase activities. -!- May also catalyze the reaction of EC 1.7.99.1 since this is a well- known activity of cytochrome cd1. -!- Formerly EC 1.6.6.5, EC 1.7.99.3 and EC 1.9.3.2.

UniProtKB Entries (1)

P24474
NIRS_PSEAE
Pseudomonas aeruginosa PAO1
Nitrite reductase

PDB Structure

PDB 1NNO
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Conformational changes occurring upon reduction and NO binding in nitrite reductase from Pseudomonas aeruginosa.
Nurizzo, D., Cutruzzola, F., Arese, M., Bourgeois, D., Brunori, M., Cambillau, C., Tegoni, M.
Biochemistry