CATH Classification

Domain Context

CATH Clusters

Superfamily 1.50.10.20
Functional Family Geranylgeranyl transferase type-1 subunit beta

Enzyme Information

2.5.1.59
Protein geranylgeranyltransferase type I.
based on mapping to UniProt P53610
Geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl- protein + diphosphate.
-!- This enzyme, along with EC 2.5.1.58 and EC 2.5.1.60, constitutes the protein prenyltransferase family of enzymes. -!- Catalyzes the formation of a thioether linkage between the C-1 atom of the geranylgeranyl group and a cysteine residue fourth from the C-terminus of the protein. -!- These protein acceptors have the C-terminal sequence CA(1)A(2)X, where the terminal residue, X, is preferably leucine; serine, methionine, alanine or glutamine makes the protein a substrate for EC 2.5.1.58. -!- The enzymes are relaxed in specificity for A(1), but cannot act if A(2) is aromatic. -!- Known targets of this enzyme include most gamma-subunits of heterotrimeric G proteins and Ras-related GTPases such as members of the Ras and Rac/Rho families. -!- The Zn(2+) is required for peptide, but not for isoprenoid, substrate binding.

UniProtKB Entries (2)

Q04631
FNTA_RAT
Rattus norvegicus
Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha
P01116
RASK_HUMAN
Homo sapiens
GTPase KRas

PDB Structure

PDB 1N4R
External Links
Method X-RAY DIFFRACTION
Organism Spodoptera
Primary Citation
Structure of mammalian protein geranylgeranyltransferase type-I
Taylor, J.S., Reid, T.S., Terry, K.L., Casey, P.J., Beese, L.S.
EMBO J.