CATH Classification

Domain Context

CATH Clusters

Superfamily 5' to 3' exonuclease, C-terminal subdomain
Functional Family DNA-directed RNA polymerase

Enzyme Information

2.7.7.6
DNA-directed RNA polymerase.
based on mapping to UniProt P00573
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
-!- Catalyzes DNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. -!- Can initiate a chain de novo. -!- In eukaryotes three forms of the enzyme have been distinguished on the basis of sensitivity of alpha-amanitin, and the type of RNA synthesized. -!- See also EC 2.7.7.19 and EC 2.7.7.48.

UniProtKB Entries (1)

P00573
RPOL_BPT7
Enterobacteria phage T7
T7 RNA polymerase

PDB Structure

PDB 1MSW
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structural Basis for the Transition from Initiation to Elongation Transcription in T7 RNA Polymerase
Yin, Y.W., Steitz, T.A.
Science