CATH Classification

Domain Context

CATH Clusters

Superfamily Methylated DNA-protein cysteine methyltransferase domain
Functional Family Methylated-DNA--protein-cysteine methyltransferase

Enzyme Information

2.1.1.63
Methylated-DNA--[protein]-cysteine S-methyltransferase.
based on mapping to UniProt O74023
(1) DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine. (2) DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.
-!- This protein is involved in the repair of methylated DNA. -!- Unlike EC 3.2.2.20 and EC 3.2.2.21, which remove the methylated base leaving an apurinic/apyrimidinic site, this enzyme transfers the methyl group from the methylated DNA to an internal cysteine residue, leaving an intact nucleotide. -!- Since the methyl transfer is irreversible, the enzyme can only catalyze a single turnover.

UniProtKB Entries (1)

O74023
OGT_THEKO
Thermococcus kodakarensis KOD1
Methylated-DNA--protein-cysteine methyltransferase

PDB Structure

PDB 1MGT
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Hyperthermostable protein structure maintained by intra and inter-helix ion-pairs in archaeal O6-methylguanine-DNA methyltransferase.
Hashimoto, H., Inoue, T., Nishioka, M., Fujiwara, S., Takagi, M., Imanaka, T., Kai, Y.
J.Mol.Biol.