CATH Classification

Domain Context

CATH Clusters

Superfamily alpha/beta hydrolase
Functional Family Pimeloyl-[acyl-carrier protein] methyl ester esterase

Enzyme Information

3.1.1.85
Pimeloyl-[acyl-carrier protein] methyl ester esterase.
based on mapping to UniProt P13001
Pimeloyl-[acyl-carrier protein] methyl ester + H(2)O = pimeloyl-[acyl- carrier protein] + methanol.
-!- Involved in biotin biosynthesis in Gram-negative bacteria. -!- The enzyme exhibits carboxylesterase activity, particularly toward substrates with short acyl chains. -!- Even though the enzyme can interact with coenzyme A thioesters, the in vivo role of the enzyme is to hydrolyze the methyl ester of pimeloyl-[acyl carrier protein], terminating the part of the biotin biosynthesis pathway that is catalyzed by the fatty acid elongation enzymes.

UniProtKB Entries (1)

P13001
BIOH_ECOLI
Escherichia coli K-12
Pimeloyl-[acyl-carrier protein] methyl ester esterase

PDB Structure

PDB 1M33
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Integrating structure, bioinformatics, and enzymology to discover function: BioH, a new carboxylesterase from Escherichia coli.
Sanishvili, R., Yakunin, A.F., Laskowski, R.A., Skarina, T., Evdokimova, E., Doherty-Kirby, A., Lajoie, G.A., Thornton, J.M., Arrowsmith, C.H., Savchenko, A., Joachimiak, A., Edwards, A.M.
J.Biol.Chem.