CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.1190.20
Functional Family

Enzyme Information

4.2.1.136
ADP-dependent NAD(P)H-hydrate dehydratase.
based on mapping to UniProt P94368
(1) ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine- dinucleotide = AMP + phosphate + NADH. (2) ADP + (6S)-6-beta-hydroxy-1,4,5,6-tetrahydronicotinamide adenine- dinucleotide phosphate = AMP + phosphate + NADPH.
-!- Acts equally well on hydrated NADH and hydrated NADPH. -!- NAD(P)H spontaneously hydrates to both the (6S)- and (6R)- isomers. -!- The enzyme from bacteria consists of two domains, one of which acts as an NAD(P)H-hydrate epimerase that interconverts the two isomers to a 60:40 ratio (cf. EC 5.1.99.6), while the other catalyzes the dehydration. -!- Hence the enzyme can restore the complete mixture of isomers into NAD(P)H. -!- The enzyme has no activity with ATP, contrary to the enzyme from eukaryotes (cf. EC 4.2.1.93).

UniProtKB Entries (1)

P94368
NNRD_BACSU
Bacillus subtilis subsp. subtilis str. 168
ADP-dependent (S)-NAD(P)H-hydrate dehydratase

PDB Structure

PDB 1KYH
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structure of Bacillus subtilis YXKO--a member of the UPF0031 family and a putative kinase.
Zhang, R., Grembecka, J., Vinokour, E., Collart, F., Dementieva, I., Minor, W., Joachimiak, A.
J.Struct.Biol.