CATH Classification

Domain Context

CATH Clusters

Superfamily NAD(P)-binding Rossmann-like Domain
Functional Family Glutathione-independent formaldehyde dehydrogenase

Enzyme Information

1.2.1.46
Formaldehyde dehydrogenase.
based on mapping to UniProt P46154
Formaldehyde + NAD(+) + H(2)O = formate + NADH.
1.2.98.1
Formaldehyde dismutase.
based on mapping to UniProt P46154
2 formaldehyde + H(2)O = formate + methanol.
-!- Enzyme-bound NADPH formed by oxidation of formaldehyde to formate is oxidized back to NADP(+) by reaction with a second formaldehyde, yielding methanol. -!- The enzyme from Mycobacterium sp. DSM 3803 also catalyzes the reactions of EC 1.1.99.36 and EC 1.1.99.37. -!- Formaldehyde and acetaldehyde can act as donors; formaldehyde, acetaldehyde and propanal can act as acceptors. -!- Formerly EC 1.2.99.4.

UniProtKB Entries (1)

P46154
FADH_PSEPU
Pseudomonas putida
Glutathione-independent formaldehyde dehydrogenase

PDB Structure

PDB 1KOL
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal Structure of Formaldehyde Dehydrogenase from Pseudomonas putida: the Structural Origin of the Tightly Bound Cofactor in Nicotinoprotein Dehydrogenases
Tanaka, N., Kusakabe, Y., Ito, K., Yoshimoto, T., Nakamura, K.T.
J.mol.biol.