CATH Classification

Domain Context

CATH Clusters

Functional Family Formamidopyrimidine-DNA glycosylase H2TH domain protein

Enzyme Information
DNA-(apurinic or apyrimidinic site) lyase.
based on mapping to UniProt P05523
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
-!- 'Nicking' of the phosphodiester bond is due to a lyase-type reaction, not hydrolysis. -!- This group of enzymes was previously listed as endonucleases, under the number EC
DNA-formamidopyrimidine glycosylase.
based on mapping to UniProt P05523
Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.
-!- May play a significant role in processes leading to recovery from mutagenesis and/or cell death by alkylating agents. -!- Also involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA.

UniProtKB Entries (1)

Escherichia coli K-12
Formamidopyrimidine-DNA glycosylase

PDB Structure

PDB 1K82
External Links
Organism Escherichia
Primary Citation
Structure of formamidopyrimidine-DNA glycosylase covalently complexed to DNA.
Gilboa, R., Zharkov, D.O., Golan, G., Fernandes, A.S., Gerchman, S.E., Matz, E., Kycia, J.H., Grollman, A.P., Shoham, G.