CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.630.30
Functional Family Glycylpeptide N-tetradecanoyltransferase

Enzyme Information

2.3.1.97
Glycylpeptide N-tetradecanoyltransferase.
based on mapping to UniProt P30418
Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal- N-tetradecanoylglycyl-[protein].
-!- The enzyme catalyzes the transfer of myristic acid from myristoyl-CoA to the amino group of the N-terminal glycine residue in a variety of eukaryotic proteins. -!- It uses an ordered Bi Bi reaction in which myristoyl-CoA binds to the enzyme prior to the binding of the peptide substrate, and CoA release precedes the release of the myristoylated peptide. -!- The enzyme from yeast is profoundly affected by amino acids further from the N-terminus, and is particularly stimulated by a serine residue at position 5.

UniProtKB Entries (1)

P30418
NMT_CANAL
Candida albicans SC5314
Glycylpeptide N-tetradecanoyltransferase

PDB Structure

PDB 1IYK
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal Structures of Candida albicans N-Myristoyltransferase with Two Distinct Inhibitors
Sogabe, S., Masubuchi, M., Sakata, K., Fukami, T.A., Morikami, K., Shiratori, Y., Ebiike, H., Kawasaki, K., Aoki, Y., Shimma, N., D'Arcy, A., Winkler, F.K., Banner, D.W., Ohtsuka, T.
CHEM.BIOL.