CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
1 | Mainly Alpha |
|
1.50 | Alpha/alpha barrel |
|
1.50.10 | Glycosyltransferase |
|
1.50.10.100 | Chondroitin AC/alginate lyase |
Domain Context
CATH Clusters
| Superfamily | Chondroitin AC/alginate lyase |
| Functional Family |
Enzyme Information
| 4.2.2.20 |
Chondroitin-sulfate-ABC endolyase.
based on mapping to UniProt P59807
Endolytic cleavage of (1->4)-beta-galactosaminic bonds between N-acetylgalactosamine and either D-glucuronic acid or L-iduronic acid to produce a mixture of Delta(4)-unsaturated oligosaccharides of different sizes that are ultimately degraded to Delta(4)-unsaturated tetra- and disaccharides.
-!- Degrades a variety of glycosaminoglycans of the chondroitin-sulfate- and dermatan-sulfate type. -!- Chondroitin sulfate, chondroitin-sulfate proteoglycan and dermatan sulfate are the best substrates but the enzyme can also act on hyaluronan at a much lower rate. -!- Keratan sulfate, heparan sulfate and heparin are not substrates. -!- In general, chondroitin sulfate (CS) and dermatan sulfate (DS) chains comprise a linkage region, a chain cap and a repeat region. -!- The repeat region of CS is a repeating disaccharide of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) [-4)GlcA(beta-1- 3)GalNAc(beta-1-](n), which may be O-sulfated on the C-4 and/or C-6 of GalNAc and C-2 of GlcA. -!- GlcA residues of CS may be epimerized to iduronic acid (IdoA) forming the repeating disaccharide [-4)IdoA(alpha-1-3)GalNAc(beta-1-](n) of DS. -!- Both the concentrations and locations of sulfate-ester substituents vary with glucosaminoglycan source. -!- The related enzyme EC 4.2.2.21 has the same substrate specificity but removes disaccharide residues from the non-reducing ends of both polymeric chondroitin sulfates and their oligosaccharide fragments produced by EC 4.2.2.20. -!- Formerly EC 4.2.2.4 and EC 4.2.99.6.
|
UniProtKB Entries (1)
| P59807 |
CABC1_PROVU
Proteus vulgaris
Chondroitin sulfate ABC endolyase
|
PDB Structure
| PDB | 1HN0 |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Crystal Structure of Proteus vulgaris Chondroitin Sulfate ABC Lyase I at 1.9 Angstroms Resolution
J.Mol.Biol.
|
