CATH Classification

Domain Context

CATH Clusters

Superfamily Polysaccharide lyase family 8-like, C-terminal
Functional Family

Enzyme Information

4.2.2.5
Chondroitin AC lyase.
based on mapping to UniProt Q59288
Eliminative degradation of polysaccharides containing 1,4-beta-D- hexosaminyl and 1,3-beta-D-glucuronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups.
-!- Acts on chondroitin 4-sulfate and chondroitin 6-sulfate, but less well on hyaluronate. -!- In general, chondroitin sulfate (CS) and dermatan sulfate (DS) chains comprise a linkage region, a chain cap and a repeat region. -!- The repeat region of CS is a repeating disaccharide of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) [-4)GlcA(beta-1- 3)GalNAc(beta-1-](n), which may be O-sulfated on the C-4 and/or C-6 of GalNAc and C-2 of GlcA. -!- GlcA residues of CS may be epimerized to iduronic acid (IdoA) forming the repeating disaccharide [-4)IdoA(alpha-1-3)GalNAc(beta-1-](n) of DS. -!- Both the concentrations and locations of sulfate-ester substituents vary with glucosaminoglycan source. -!- Formerly EC 4.2.99.6.

UniProtKB Entries (1)

Q59288
CSLA_PEDHD
Pedobacter heparinus DSM 2366
Chondroitinase-AC

PDB Structure

PDB 1HM2
External Links
Method X-RAY DIFFRACTION
Organism Pedobacter
Primary Citation
Active site of chondroitin AC lyase revealed by the structure of enzyme-oligosaccharide complexes and mutagenesis.
Huang, W., Boju, L., Tkalec, L., Su, H., Yang, H.O., Gunay, N.S., Linhardt, R.J., Kim, Y.S., Matte, A., Cygler, M.
Biochemistry