CATH Classification

Domain Context

CATH Clusters

Superfamily Quinoprotein alcohol dehydrogenase-like superfamily
Functional Family Methanol dehydrogenase large subunit

Enzyme Information
Methanol dehydrogenase (cytochrome c).
based on mapping to UniProt P16027
A primary alcohol + 2 cytochrome c(L) = an aldehyde + 2 reduced cytochrome c(L) + 2 H(+).
-!- A periplasmic quinoprotein alcohol dehydrogenase that only occurs in methylotrophic bacteria. -!- It uses the novel specific cytochrome c(L) as acceptor. -!- Acts on a wide range of primary alcohols, including ethanol, duodecanol, chloroethanol, cinnamyl alcohol, and also formaldehyde. -!- Activity is stimulated by ammonia or methylamine. -!- It is usually assayed with phenazine methosulfate. -!- Like all other quinoprotein alcohol dehydrogenases it has an 8-bladed 'propeller' structure, a calcium ion bound to the PQQ in the active site and an unusual disulfide ring structure in close proximity to the PQQ. -!- It differs from EC, alcohol dehydrogenase (cytochrome c), in having a high affinity for methanol and in having a second essential small subunit (no known function). -!- Formerly EC

UniProtKB Entries (1)

Methylobacterium extorquens AM1
Methanol dehydrogenase [cytochrome c] subunit 1

PDB Structure

External Links
Primary Citation
The Refined Structure of the Quinoprotein Methanol Dehydrogenase from Methylobacterium Extorquens at 1.94 A.
Ghosh, M., Anthony, C., Harlos, K., Goodwin, M.G., Blake, C.