CATH Classification

Domain Context

CATH Clusters

Superfamily 2.70.40.10
Functional Family

Enzyme Information

2.7.7.49
RNA-directed DNA polymerase.
based on mapping to UniProt P16088
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
-!- Catalyzes RNA-template-directed extension of the 3'-end of a DNA strand by one deoxynucleotide at a time. -!- Cannot initiate a chain de novo. -!- Requires a RNA or DNA primer. -!- DNA can also serve as template. -!- See also EC 2.7.7.7.
3.1.13.2
Exoribonuclease H.
based on mapping to UniProt P16088
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
-!- This is a secondary reaction to the RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end performed by EC 3.1.26.13.
3.1.26.13
Retroviral ribonuclease H.
based on mapping to UniProt P16088
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
-!- Retroviral reverse transcriptase is a multifunctional enzyme responsible for viral replication. -!- To perform this task the enzyme combines two distinct activities. -!- The polymerase domain (EC 2.7.7.49) occupies the N-terminal two- thirds of the reverse transcriptase whereas the ribonuclease H domain comprises the C-terminal remaining one-third. -!- The RNase H domain of Moloney murine leukemia virus and Human immunodeficiency virus display two metal binding sites.
3.4.23.-
Aspartic endopeptidases.
based on mapping to UniProt P16088
3.6.1.23
dUTP diphosphatase.
based on mapping to UniProt P16088
dUTP + H(2)O = dUMP + diphosphate.
3.1.-.-
Acting on ester bonds.
based on mapping to UniProt P16088
2.7.7.-
Nucleotidyltransferases.
based on mapping to UniProt P16088

UniProtKB Entries (1)

P16088
POL_FIVPE
Feline immunodeficiency virus (isolate Petaluma)
Pol polyprotein

PDB Structure

PDB 1F7O
External Links
Method X-RAY DIFFRACTION
Organism Trichoplusia
Primary Citation
Structures of feline immunodeficiency virus dUTP pyrophosphatase and its nucleotide complexes in three crystal forms.
Prasad, G.S., Stura, E.A., Elder, J.H., Stout, C.D.
Acta Crystallogr.,Sect.D