CATH Classification

Domain Context

CATH Clusters

Superfamily Uracil-DNA glycosylase-like domain
Functional Family Uracil-DNA glycosylase

Enzyme Information

3.2.2.27
Uracil-DNA glycosylase.
based on mapping to UniProt P13051
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.
-!- Uracil-DNA glycosylases are widespread enzymes that are found in all living organisms. -!- Uracil-DNA glycosylase (EC 3.2.2.27) and double-stranded uracil-DNA glycosylase (EC 3.2.2.28) form a central part of the DNA-repair machinery since they initiate the DNA base-excision repair pathway by hydrolyzing the N-glycosidic bond between uracil and the deoxyribose sugar thereby catalyzing the removal of mis-incorporated uracil from DNA.

UniProtKB Entries (1)

P13051
UNG_HUMAN
Homo sapiens
Uracil-DNA glycosylase

PDB Structure

PDB 1EMH
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Uracil-DNA glycosylase-DNA substrate and product structures: conformational strain promotes catalytic efficiency by coupled stereoelectronic effects.
Parikh, S.S., Walcher, G., Jones, G.D., Slupphaug, G., Krokan, H.E., Blackburn, G.M., Tainer, J.A.
Proc.Natl.Acad.Sci.USA