CATH Classification

Domain Context

CATH Clusters

Superfamily alpha/beta hydrolase
Functional Family Bifunctional epoxide hydrolase 2

Enzyme Information

3.1.3.76
Lipid-phosphate phosphatase.
based on mapping to UniProt P34914
(9S,10S)-10-hydroxy-9-(phosphonooxy)octadecanoate + H(2)O = (9S,10S)- 9,10-dihydroxyoctadecanoate + phosphate.
-!- The enzyme from mammals is a bifunctional enzyme: the N-terminal domain exhibits lipid-phosphate-phosphatase activity and the C-terminal domain has the activity of EC 3.3.2.10. -!- The best substrates for this enzyme are 10-hydroxy-9- (phosphonooxy)octadecanoates, with the threo- form being a better substrate than the erythro- form. -!- The phosphatase activity is not found in plant EC 3.3.2.10 or in mammalian EC 3.3.2.9.
3.3.2.10
Soluble epoxide hydrolase.
based on mapping to UniProt P34914
An epoxide + H(2)O = a glycol.
-!- Catalyzes the hydrolysis of trans-substituted epoxides, such as trans-stilbene oxide, as well as various aliphatic epoxides derived from fatty-acid metabolism. -!- It is involved in the metabolism of arachidonic epoxides (epoxyeicosatrienoic acids; EETs) and linoleic acid epoxides. -!- The enzyme from mammals is a bifunctional enzyme: the C-terminal domain exhibits epoxide-hydrolase activity and the N-terminal domain has the activity of EC 3.1.3.76. -!- Like EC 3.3.2.9, it is probable that the reaction involves the formation of an hydroxyalkyl-enzyme intermediate. -!- The enzyme can also use leukotriene A(4), the substrate of EC 3.3.2.6, but it forms 5,6-dihydroxy-7,9,11,14-eicosatetraenoic acid rather than leukotriene B(4) as the product. -!- Formerly EC 3.3.2.3, EC 4.2.1.63 and EC 4.2.1.64.

UniProtKB Entries (1)

P34914
HYES_MOUSE
Mus musculus
Bifunctional epoxide hydrolase 2

PDB Structure

PDB 1EK2
External Links
Method X-RAY DIFFRACTION
Organism Spodoptera
Primary Citation
Binding of alkylurea inhibitors to epoxide hydrolase implicates active site tyrosines in substrate activation.
Argiriadi, M.A., Morisseau, C., Goodrow, M.H., Dowdy, D.L., Hammock, B.D., Christianson, D.W.
J.Biol.Chem.