CATH Classification

Domain Context

CATH Clusters

Superfamily B-lactam Antibiotic, Isopenicillin N Synthase; Chain
Functional Family

Enzyme Information

1.14.11.28
Proline 3-hydroxylase.
based on mapping to UniProt O09345
L-proline + 2-oxoglutarate + O(2) = cis-3-hydroxy-L-proline + succinate + CO(2).
-!- Unlike the proline hydroxylases involved in collagen biosynthesis (EC 1.14.11.2 and EC 1.14.11.7), this enzyme does not require ascorbate for activity although it does increase the activity of the enzyme. -!- The enzyme is specific for L-proline as D-proline, trans-4-hydroxy-L- proline, cis-4-hydroxy-L-proline and 3,4-dehydro-DL-proline are not substrates.

UniProtKB Entries (1)

O09345
P3H2_STRSQ
Streptomyces sp.
L-proline cis-3-hydroxylase 2

PDB Structure

PDB 1E5R
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structure of proline 3-hydroxylase. Evolution of the family of 2-oxoglutarate dependent oxygenases.
Clifton, I.J., Hsueh, L.C., Baldwin, J.E., Harlos, K., Schofield, C.J.
Eur.J.Biochem.