CATH Classification

Domain Context

CATH Clusters

Superfamily NAD(P)-binding Rossmann-like Domain
Functional Family Bifunctional protein FolD

Enzyme Information

6.3.4.3
Formate--tetrahydrofolate ligase.
based on mapping to UniProt P11586
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.
-!- In eukaryotes occurs as a trifunctional enzyme also having EC 1.5.1.5 and EC 3.5.4.9 activity.
3.5.4.9
Methenyltetrahydrofolate cyclohydrolase.
based on mapping to UniProt P11586
5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate.
1.5.1.5
Methylenetetrahydrofolate dehydrogenase (NADP(+)).
based on mapping to UniProt P11586
5,10-methylenetetrahydrofolate + NADP(+) = 5,10- methenyltetrahydrofolate + NADPH.

UniProtKB Entries (1)

P11586
C1TC_HUMAN
Homo sapiens
C-1-tetrahydrofolate synthase, cytoplasmic

PDB Structure

PDB 1DIA
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Structures of three inhibitor complexes provide insight into the reaction mechanism of the human methylenetetrahydrofolate dehydrogenase/cyclohydrolase.
Schmidt, A., Wu, H., MacKenzie, R.E., Chen, V.J., Bewly, J.R., Ray, J.E., Toth, J.E., Cygler, M.
Biochemistry